Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis

26 de abril de 2020

Alterações de aminoácidos na proteína Spike do coronavírus felino correlacionam-se com a disseminação sistêmica de vírus do intestino e não com peritonite infecciosa felina

Autores

Emily Porter, Séverine Tasker,Michael J Day, Ross Harley, Anja Kipar, Stuart G Siddell,Christopher R Helps

Abstract

Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results in anamino acid change from methionine to leucine at position 1058, may be associated with feline infectious peritonitis(FIP). Tissue and faecal samples collected post mortem from cats diagnosed with or without FIP were subjected to RNA extraction and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to detect FCoV RNA. In cats withFIP, 95% of tissue, and 81% of faecal samples were PCR-positive, as opposed to 22% of tissue, and 60% of faecal samples in cats without FIP. Relative FCoV copy numbers were significantly higher in the cats with FIP, both intissues (P< 0.001) and faeces (P= 0.02). PCR-positive samples underwent pyrosequencing encompassing position 1058 of the FCoV spike protein. This identified a methionine codon at position 1058, consistent with the shedding of an enteric form of FCoV, in 77% of the faecal samples from cats with FIP, and in 100% of the samples from cats without FIP. In contrast, 91% of the tissue samples from cats with FIP and 89% from cats without FIP had a leucine codon at position 1058, consistent with a systemic form of FCoV. These results suggest that the methionine to leucine substitution at position 1058 in the FCoV spike protein is indicative of systemic spread of FCoV from the intestine, ratherthan a virus with the potential to cause FIP.

 

 

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