World Small Animal Veterinary Association Renal Pathology Initiative: Classification of Glomerular Diseases in Dogs

21 de fevereiro de 2020

Iniciativa da Associação Mundial de Patologia Renal Veterinária de Pequenos Animais: Classificação de Doenças Glomerulares em Cães

Autores

R. E. Cianciolo, F. C. Mohr, L. Aresu,  C. A. Brown,  C. James, J. H. Jansen, W. L. Spangler, J. J. van der Lugt, P. H. Kass, C. Brovida, L. D. Cowgill, R. Heiene, D. J. Polzin, H. Syme, S. L. Vaden, A. M. van Dongen, G. E. Lees

Abstract

Evaluation of canine renal biopsy tissue has generally relied on light microscopic (LM) evaluation of hematoxylin and eosin–stained sections ranging in thickness from 3 to 5mm. Advanced modalities, such as transmission electron microscopy (TEM)and immunofluorescence (IF), have been used sporadically or retrospectively. Diagnostic algorithms of glomerular diseaseshave been extrapolated from the World Health Organization classification scheme for human glomerular disease. With therecent establishment of 2 veterinary nephropathology services that evaluate 3-mm sections with a panel of histochemicalstains and routinely perform TEM and IF, a standardized objective species-specific approach for the diagnosis of canineglomerular disease was needed. Eight veterinary pathologistsevaluated 114 parameters (lesions) in renal biopsy specimensfrom 89 dogs. Hierarchical cluster analysis of the data revealed 2 large categories of glomerular disease based on thepresence or absence of immune complex deposition: The immune complex–mediated glomerulonephritis (ICGN) categoryincluded cases with histologic lesions of membranoproliferative or membranous patterns.The second category includedcontrol dogs and dogs with non-ICGN (glomerular amyloidosisor focal segmental glomerulosclerosis). Cluster analysisperformed on only the LM parameters led to misdiagnosis of22 of the 89 cases—that is, ICGN cases moved to the non-ICGN branch of the dendrogram or vice versa, thereby emphasizing the importance of advanced diagnostic modalities inthe evaluation of canine glomerular disease. Salient LM, TEM, and IF features for each pattern of disease were identified,and a preliminary investigation of related clinicopathologic data was performed.

Keywords

glomerulopathies, glomerulonephritis, proteinuria

Palavras chave

glomerulopatias, glomerulonefrite, proteinúria

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