Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs

6 de agosto de 2020

Validação de teste rápido de ELISA para diagnóstico sorológico de Leishmania chagasi em cães

Autores

M. Marcondes,  A.W. Biondo, A.A.D. Gomes,  A.R.S. Silva,  R.F.C. Vieira, A.A. Camacho, John Quinng, R. Chandrashekar

Abstract

Canine visceral leishmaniasis (CVL) is caused by Leishmania donovani complex parasites
including L. donovani, Leishmania infantum and Leishmania chagasi. As some studies suggest
that L. chagasi and L. infantum may be very similar or even the same species, the aim of the
present study was to evaluate a commercial rapid ELISA test, originally designed for L. infantum, in the diagnosis of CVL in dogs naturally infected by L. chagasi. A total of 400 serum
canine samples, including 283 positive dogs for CVL from an endemic area, 86 clinically
healthy dogs from a non-endemic area and 31 dogs seropositive for confounding infectious
agents (Trypanosoma cruzi, Toxoplasma gondii, Neospora caninum, Babesia canis and Ehrlichia
canis) were used for test validation. An overall sensitivity of 94.7% (95% CI = 91.41–97.01%)
and specificity of 90.6% (95% CI = 83.80–95.21%) was found, with a high degree of agreement
(k = 0.8445) to the indirect ELISA. When confounding infectious diseases were excluded,
specificity increased to 100% (95% CI = 95.8–100%), with a higher degree of agreement
(k = 0.8928). In conclusion, the commercial kit designed for L. infantum was a highly sensitive and specific device for detection of L. chagasi infection in dogs, which indicates high
immunoreactivity similarities between L. infantum and L. chagasi

Keywords

Visceral leishmaniasis, Serology, Snap

Palavras-chave

Leishmaniose visceral, Sorologia, Snap

 

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