TESA-blot for the diagnosis of Chagas disease in dogs from co-endemic regions for Trypanosoma cruzi, Trypanosoma evansi and Leishmania chagasi

7 de agosto de 2020

TESA-blot para diagnóstico da doença de Chagas em cães de regiões co-endêmicas de Trypanosoma cruzi, Trypanosoma evansi e Leishmania chagasi

Autores

E.S. Umezawa, A.I. Souza, V. Pinedo-Cancino, M. Marcondes , A. Marcili , L.M.A. Camargo, A.A. Camacho, A.M.S. Stolf, M.M.G. Teixeira

Abstract

We standardized serodiagnosis of dogs infected with Trypanosoma cruzi using TESA (trypomastigote excreted-secreted antigen)-blot developed for human Chagas disease. TESA-blot showed 100% sensitivity and specificity. In contrast, ELISA using TESA (TESA-ELISA) or epimastigotes (epi-ELISA) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. When used in field studies in an endemic region for Chagas disease, visceral leishmaniasis and Trypanosoma evansi (Mato Grosso do Sul state, Central Brazil), positivities were 9.3% for TESA-blot, 10.7% for TESA-ELISA and 32% for epi-ELISA. Dogs from a non-endemic region for these infections (Rondonia state, western Amazonia) where T. cruzi is enzootic showed positivity of 4.5% for TESA-blot and epi-ELISA and 6.8% for TESA-ELISA. Sera from
urban dogs from Santos, São Paulo, where these diseases are absent, yielded negative results. TESA-blot was the only method that distinguished dogs infected with T. cruzi from those infected with Leishmania chagasi and/or Trypanosoma evansi.

Keywords

Trypanosoma cruzi, Canine Chagas disease, TESA-blot, Serological diagnosis, Dogs, Leishmania chagasi, Trypanosoma evansi

Palavras-chave

Trypanosoma cruzi, Doença de Chagas canina, TESA-blot, Diagnóstico sorológico, Cães, Leishmania chagasi, Trypanosoma evansi

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