MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL

19 de novembro de 2020

Investigação molecular de micoplasma hemotrópico em seres humanos, cães e cavalos em região rural do sul do Brasil.

Rafael Felipe da Costa VIEIRA, Odilon VIDOTTO, Thállitha Samih Wischral Jayme VIEIRA, Ana Márcia Sá GUIMARAES, Andrea Pires dos SANTOS(3), Naíla Cannes NASCIMENTO, Nelson Jesse Rodrigues dos SANTOS, Thiago Fernandes MARTINS, Marcelo Bahia LABRUNA, Mary MARCONDES, Alexander Welker BIONDO, & Joanne Belle MESSICK

Abstract

The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement’s population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanis alone, 12 ‘Candidatus Mycoplasma haematoparvum’ alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas
(p = 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.

Keywords

Hemoplasma; Mycoplasma haemocanis; ‘Candidatus Mycoplasma haematoparvum’; Real-time PCR.

Palavras chave:

Hemoplasma, Micoplasma, PRC real- time

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