Clinical performance of a point-of-care Coccidioides antibody test in dogs

11 de março de 2021

Performance clínica do teste de anticorpos para Coccidioides em cães realizado em ponto de atendimento.

Autores

Krystle L. Reagan, Ian McHardy, George R. Thompson III, Jane E. Sykes

Abstract

Background: Point-of-care (POC) Coccidioides antibody assays may provide veterinarians with rapid and accurate diagnostic information.
Objectives: To determine the agreement of a POC lateral flow assay (LFA), sona Coccidioides (IMMY, Norman, Oklahoma) with the current diagnostic standard, the immunodiffusion assay (agar gel immunodiffusion [AGID]; Coccidioidomycosis Serology Laboratory, University of California, Davis, California).
Animals: Forty-eight sera specimens from 48 dogs.
Methods: Sera specimens were collected from client-owned dogs that had a clinical suspicion for coccidioidomycosis. Animals were classified as Coccidioides antibodypositive (n = 36) based on a positive AGID or Coccidioides antibody-negative (n = 12) based on a negative AGID. The performance of the LFA assay was determined by comparing results to AGID results.
Results: The LFA assay demonstrated agreement in 32 of 36 Coccidioides antibodypositive specimens and 12 of 12 Coccidioides antibody-negative specimens, resulting in a positive percentage agreement of 88.9% (95% confidence interval [CI], 74.7-95.6%) and negative percentage agreement of 100% (95% CI, 75.8-100%) as compared to AGID. A receiver operator characteristic curve was constructed, and the area under the curve was 0.944 (CI, 0.880-1.000).
Conclusion and Clinical importance: This LFA is a rapid alternative to the traditional AGID. The LFA provides excellent predictive value for positive results. Positive agreement was lower in dogs with low AGID titers; therefore, confirmatory testing is recommended if a high index of suspicion exists.

Keywords

Diagnostic test, fungal, lateral flow, veterinary

Palavras chaves

Teste diagnóstico, fungos, fluxo lateral, veterinária

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